What is the process of peptide synthesis?
In organic chemistry, peptide synthesis is the production of peptides, compounds where multiple amino acids are linked via amide bonds, also known as peptide bonds. Peptides are chemically synthesized by the condensation reaction of the carboxyl group of one amino acid to the amino group of another.
What is Merrifield synthesis?
Merrifield solid phase peptide synthesis has been the principle research procedure used in the study of the chemistry and biological use of deamidation of asparaginyl and glutaminyl residues in peptides and proteins during the past 40 years.
How is the peptide chain synthesized?
Peptides are synthesized by coupling the carboxyl group or C-terminus of one amino acid to the amino group or N-terminus of another. Due to the possibility of unintended reactions, protecting groups are usually necessary. Chemical peptide synthesis starts at the C-terminal end of the peptide andends at the N-terminus.
Who discovered the solid phase peptide synthesis?
Bruce Merrifield
Inventor of solid-phase peptide synthesis. The death of Bruce Merrifield on 14 May 2006 brings to a close the life of one of the most original scientists of the second half of the twentieth century. Between 1959 and 1963, Merrifield revolutionized organic chemistry by his invention of solid-phase peptide synthesis.
What is the significance of Ramachandran plot?
The Ramachandran plot provides a way to view the distribution of torsion angles in a protein structure and shows that the torsion angles corresponding to the two major secondary structure elements (α-helices and β-sheets) are clearly clustered within separate regions.
What are the roles of Merrifield resin?
Merrifield Resin is a cross-linked polystyrene resin that carries a chloromethyl functional group. Merrifield resin is named after its inventor, Robert Bruce Merrifield (1984 winner of the Nobel Prize in Chemistry), and used in solid-phase synthesis. The material is typically available as white beads.
What are synthetic peptides?
Synthetic peptides are chemically synthesized small polymers of amino acids. You can think of synthetic peptides as being to proteins what oligos are to DNA.
What was the first peptide reported?
The concept is a straightforward one, whereby peptide elongation proceeds via a coupling reaction between amino acids, followed by removal of a reversible protecting group. The first peptide synthesis, as well as the creation of the term “peptide,” was reported by Fischer and Fourneau (Fischer and Fourneau, 1901).
What is resin in peptide synthesis?
Polystyrene. Polystyrene is the most common core resin in solid phase peptide synthesis, but other core matrices include polyacrylate, polyacrylamide, and polyethylene glycol.