Are BamHI and BglII compatible?
BamHI and BglII generate different-but-compatible ends. Ligation is possible between both identical ends and between the different-but-compatible ends, but identical-end products will be converted back into substrate by BamHI and BglII digestion.
What ends are produced by Hind III?
Option B: Hind 3: It is a type 2 restriction endonuclease which gives sticky ends. It is isolated from Haemophilus influenzae.
Does BamHI produce sticky or blunt ends?
BamHI binds at the recognition sequence 5′-GGATCC-3′, and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in sticky ends which are 4 bp long.
Which two enzymes create cut sites that could be ligated together?
The enzymes BglII (1) and Sau3A (3) will generate compatible ends that can be ligated together by DNA ligase.
What does BamHI cleave?
BamHI binds at the recognition sequence 5′-GGATCC-3′ , and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in “sticky ends” which are 4 b.p. long. In its unbound form, BamHI displays a central b sheet, which resides in between a helices .
What type of ends do the enzymes BamHI and EcoRI produce?
EcoRI – recognises the sequence 5’GAATTC’3 – sticky ends. BamHI – recognises the sequence 5’GGATCC’3 – sticky ends.
What is a unit of BamHI?
A E. coli strain that carries the BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). One unit is defined as the amount of BamHI required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl..
Where can I find more details about BSA-free versions of BamHI?
Find more details at www.neb.com/BSA-free. BamHI has a High Fidelity version BamHI-HF ® ( NEB #R3136 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.
What is the conformational change of BamHI?
BamHI undergoes a series of unconventional conformational changes upon DNA recognition. This allows the DNA to maintain its normal B-DNA conformation without distorting to facilitate enzyme binding. BamHI is a symmetric dimer. DNA is bound in a large cleft that is formed between dimers; the enzyme binds in a “crossover” manner.
What is 1 unit of BglII?
One unit is defined as the amount of BglII required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Not sensitive to CpG, dcm, or dam methylation.