How does Tet repressor work?
TetR represses the expression of TetA, a membrane protein that pumps out substances toxic to the bacteria like Tc, by binding the tetA operator. In Tc-resistant bacteria, TetA will pump out Tc before it can bind to the ribosome because the repressive action of TetR on TetA is halted by binding of Tc to TetR.
What is the Tet operator?
The TRE is made up of Tet operator (tetO) sequence concatemers fused to a minimal promoter, (commonly the minimal promoter sequence derived from the human cytomegalovirus (hCMV) immediate-early promoter). In the absence of Tc or Dox, tTA binds to the TRE and activates transcription of the target gene.
How does the Tet operon work?
(A) Tn10 tet operon. In E. coli, TetR binds as a dimer to the tetO1 and tetO2 sites in the Tn10 tet operon. This interaction blocks the activity of the underlying promoters (PA, PR1 and PR2) and inhibits transcription of the tetA and tetR genes.
What is Tet promoter?
Tetracycline-controlled gene expression is based upon the mechanism of resistance to tetracycline antibiotic treatment found in gram-negative bacteria. In nature, the Ptet promoter expresses TetR, the repressor, and TetA, the protein that pumps tetracycline antibiotic out of the cell.
How is doxycycline different from tetracycline?
Tetracyclines are broad spectrum antibiotics often used to treat skin, chest, urethral, and pelvic infections. Doxycycline is indicated in a wide range of infections including syphilis, Lyme disease, Q fever, Rocky Mountain spotted fever, and plague. It is also widely used for malaria prophylaxis.
What is Tet-on plasmid?
The Tet system is a conditional gene expression system where transcription is turned on or off in the presence of tetracycline or doxycycline. It affords very tight control over expression compared to other inducible systems and is reversible.
What is Tet-on 3G?
Tet-On 3G tetracycline-inducible gene expression systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available.
Why is doxycycline preferred over tetracycline?
It has the advantage over other members of the tetracycline family of improved oral absorption and a prolonged serum half-life. Doxycycline has activity against a very broad range of Gram-positive, Gram-negative and ‘atypical’ bacteria as well as some protozoa such as malaria.
Are there two types of doxycycline?
Doxycycline monohydrate and doxycycline hyclate are two generic prescription antibiotics used to treat various bacterial infections.