What is alkaline phosphatase assay used for?
Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone. The Alkaline Phosphatase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure ALP activity in serum and biological samples.
How is ALP activity calculated?
ALP activity (U/ml) = A/V/T Glycine Units: The amount of enzyme causing the hydrolysis of one micromole of pNPP per minute at pH 9.6 and 25°C (glycine buffer).
What is ALP activity?
The activity of alkaline phosphatase (ALP) is considered to indicate the presence of osteoblast cells and the formation of new bone.
What is the range of ALP activity in patients suffering from hepatobiliary diseases?
The reference range with most commonly used methods is 0 to 50 IU/L in males and 0 to 30 IU/L in females. Higher activity in males is probably caused by high enzyme concentration in prostatic tissue.
What is the normal range for alk phos?
Normal Results The normal range is 44 to 147 international units per liter (IU/L) or 0.73 to 2.45 microkatal per liter (µkat/L). Normal values may vary slightly from laboratory to laboratory. They also can vary with age and sex.
What is ALP normal range?
The normal range is 44 to 147 international units per liter (IU/L) or 0.73 to 2.45 microkatal per liter (µkat/L). Normal values may vary slightly from laboratory to laboratory. They also can vary with age and sex. High levels of ALP are normally seen in children undergoing growth spurts and in pregnant women.
What is serum alkaline phosphatase level?
The normal range of alkaline phosphatase in the blood is 20 to 140U/L, although this can vary from lab to lab. Children and pregnant women can have significantly higher levels of the enzyme in their blood [1]. Values above 130 U/L are usually considered to be high.
What is normal ALP level?
What is the role of alkaline phosphatase in liver?
ALP is an enzyme found in your bloodstream. It helps break down proteins in the body and exists in different forms, depending on where it originates. Your liver is one of the main sources of ALP, but some is also made in your bones, intestines, pancreas, and kidneys. In pregnant people, ALP is made in the placenta.
What is Alk Phos in blood test high?
An alkaline phosphatase (ALP) test measures the amount of ALP in your blood. Although ALP exists throughout your body, the two main sources of ALP in your blood are your liver and bones. High levels of ALP may indicate liver disease or certain bone disorders, but an ALP test alone cannot diagnose a condition.