What is fluorometer used for?
A fluorometer (and fluorimeter) measures the fluorescence or light emitted by different fluorescing objects. Fluorescence occurs when light of specific wavelength hits and excites electrons in a sample, and the electrons in that sample instantly emit or fluoresce light of a different wavelength.
Is a fluorometer a spectrophotometer?
A fluorescence spectrophotometer (or fluorometer, fluorospectrometer, or fluorescence spectrometer) measures the fluorescent light emitted from a sample at different wavelengths, after illumination with a xenon flash lamp.
What is the instrumentation of fluorimetry?
Introduction to Instrumentation The basic components of fluorescence spectrometers are a white light source, excitation monochromator, sample chamber, emission monochromator, and detector. Advanced instruments can be configured with multiple sources, detectors and sample holders.
What are the components of a fluorometer?
Components of Fluorometers and Spectrophotofluorometers Instruments designed for fluorescence measurement have the following basic components: a light source, an excitation (primary) monochromator, a cuvette, an emission (secondary) monochromator, and a photodetector (Fig. 4.14).
Why is fluorometer sensitive?
The reason for the high sensitivity of fluorescence techniques is that the emission signal is measured above a low background level. This is inherently more sensitive than comparing two relatively large signals as in absorption spectroscopy.
What are the components of fluorometer?
What is detector in fluorometer?
Fluorometers are routinely used in molecular and cell biology laboratories to detect and quantify fluorescent light signals emitted at different wavelengths. Fluorescent compounds emit light of a particular wavelength upon stimulation or excitation.
Which detector is used in fluorimetry?
photomultiplier tube
In fluorimetry, a photomultiplier tube is used to detect the emitted fluorescence. A photomultiplier tube or PMT is a type of vacuum phototube. It is a highly sensitive detector of light in the ultraviolet, visible, and near-infrared ranges of the electromagnetic spectrum.
What is the principle of fluorimetry?
Principle of Fluorimetry: When molecules are irradiated with light of the appropriate frequency, it will be absorbed in about 10-15 seconds. In the process of absorption, the molecules may move from ground to the first excited singlet electronic state.
Who invented the fluorometer?
Clair Loring Farrand
Clair Loring Farrand (1895-1981), an inventor best known for his work with the cone loudspeaker, began his career as a Marconi radio operator.
What is a fluorescence spectrophotometer?
These industry-leading fluorescence spectrophotometers cover any measurement mode or type of data analysis your application requires: Excitation, emission, constant wavelength synchronous and constant energy synchronous spectral scanning
What is a fluorometer?
Our fluorometers help to simplify fluorescence analysis while conserving precious sample and time. Using specific wavelength Ex/Em optical filter sets (fluorometer) or Using a grating monochromator (fluorospectrometer) to select the excitation wavelength and scan the spectrum to record emission intensity versus wavelength.
Why choose the FL 6500 fluorescence spectrophotometer?
For your most sensitive samples, the FL 6500 fluorescence spectrometer provides adjustable peak power for greater dynamic range. The high-energy pulsed Xenon light source enables you to get the most accurate results without photobleaching your sample.
Why DS-11 spectrophotometers and fluorometers?
The multi-award winning DS-11 Series of 1 µL Spectrophotometers and Fluorometers provides the most sensitive and widest dynamic range for UV-Vis absorbance and fluorescence capability in one combined instrument. Selecting the appropriate quantification methods for biological samples is an important consideration in any quality control process.