What is Geltrex?
Geltrex™ is a reduced growth factor basement membrane extract used for attachment and maintenance of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs).
What is Geltrex made of?
The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
How do you dissolve Geltrex?
Using cell recovery solution:
- Wash cells thrice with cold 1x PBS and add equal amounts of cell recovery solution (227µl per cm2) directly to T25 flasks.
- Scrape the cells using cell scrapers and transfer to a cold 50 ml centrifuge tube.
- Incubate on ice till GeltrexTM is dissolved (up to 1 h, varies for each cell line).
What does Ldev-free mean?
LDEV-free = free of viruses, including lactose dehydrogenase elevating virus or LDEV.
How do you use Geltrex?
Mix Geltrex by slowly pipetting solution up and down; be careful not to introduce air bubbles. Place 150-200 µl per cm2 onto the growth surface. Place coated object at 37°C for 30 minutes. Coated objects are ready for use.
What is basement membrane extract?
Basement membrane extracts are formulations that mimic the extracellular matrix for cell culture purposes. Basement membranes in cells are thin but densely packed sheets of specialized extracellular matrix used to maintain cell integrity and structural support, while influencing cell proliferation and survival.
Does Matrigel expire?
Pre-screened for compatibility with mTeSR®1 medium by Stem Cell Technologies, providing the reproducibility and consistency essential for human embryonic and induced pluripotent feeder-free stem cell culture. 2 years from date of manufacture. Date of expiration is located on a lot-specific certificate of analysis.
Can Matrigel be reused?
Diluted Matrigel solution should not be stored and reused. Critical: Minimize the time the bottle will be kept out of the 4°C in order to avoid gelation of the Matrigel. Mix the Matrigel/DMEM solution by inversion.
How do you store Geltrex?
For long term storage, seal each dish with parafilm to minimize dehydration of the Geltrex solution. The coated dish is stable for two weeks when stored at 2 to 8°C. Do not allow coated surface to dry out and maintain a storage temperature of 2 to 8°C to avoid premature gelling.
How do you make Geltrex?
Mix Geltrex by slowly pipetting solution up and down; be careful not to introduce air bubbles. Dilute 1 ml of Geltrex into 29 ml DMEM/F12 medium (SKU: 10565). Empirical determination of the optimal coating concentration for your application may be required. Volumes can be adjusted accordingly.
Is geltrex™ virus free?
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free.
What is geltrex used for?
It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors.
What is geltrex coating for cell culture?
Cell culture dished were coated with Geltrex before seeding. Geltrex™ is a reduced growth factor basement membrane extract used for attachment and maintenance of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs).
Why is geltrex used for STEMPRO media?
Therefore, Geltrex has been used for StemPro media which is a fully-defined, serum- and feeder-free medium (SFM) specially formulated for the growth and expansion of hES and hiPSCs. Especially when cells are grown with StemPro media, cell culture dishes should be coated with Geltrex.