What is ion chromatography analysis?
Ion Chromatogram. Ion chromatography (IC) is the separation and quantitative analysis of anions and cations in an ionic solution using the ion exchange method of liquid chromatography (LC). The chromatographic process separates the different ions within the sample.
How do you analyze silica?
When analyzing for crystalline silica content, concentration is not measured directly by the laboratory. Rather, the mass of crystalline silica present in the sample is measured. The concentration is calculated based on the measured value relative to the original mass of the sample.
What is the principle of ion chromatography?
Ion chromatography (or ion-exchange chromatography) separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids.
Which are the two types of ion chromatography method?
The two types of ion chromatography are anion-exchange and cation-exchange.
What are the advantages of ion chromatography?
The most important advantages of ion chromatography are a broad range of applications, well developed hardware, many detection options, reliability with good accuracy and precision, high selectivity, high speed, high separation efficiency, good tolerance to sample matrices, and low cost of consumables.
How is silica content measured?
It is possible to measure the amount of silica in limestone aggregates using x ray–fluorescence (XRF) spectroscopy. This measurement can also be taken using a scanning electron microscope (SEM). With this technique, the electron beam in the SEM scans the surface of the aggregate and produces maps.
Which method is used for estimation of silica from any ore?
One method is available for Fly ash. First silicates are selectively dissolved with Hydroflosilicic acid leaving the free silica. In the second step free silica is determined by hydroflorization with HF.
What are the disadvantages of ion chromatography?
Disadvantage of Ion exchange chromatography
- This technique is only limited to separation of charged ions or polar molecules.
- The resin used in this technique may get damaged by some materials.
- It requires large volume of sample.
- Instrument is expensive and requires expensive chemicals such as buffers.
What are the advantages and disadvantages of ion-exchange chromatography?
Pros and Cons of Ion Exchange Chromatography
|IEX Pros||IEX Cons|
|Permits high flow rate||Sample must be loaded at low ionic strength|
|Concentrates samples||Clusters of positively charged residues can cause a net-negatively charged protein to bind a cation exchanger, and vice versa|