What is pour plate method?
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar medium, a larger volume can be used than with the spread plate.
What is pour streak plate technique?
The main difference between streak plate and pour plate is that in streak plate, the first to be added is the melted nutrient agar and the second to be added is a loop of bacteria from a slant, whereas the first to be added in pour plate is the bacterial broth and the second to be added is the nutrient agar.
Which is better pour plate or spread plate?
With regard to the accuracy of these two techniques, pour plate has a higher accuracy than the spread plate. Moreover, unlike in a pour plate, a glass spreader is used to spread the sample evenly on the surface on a spread plate.
Why spread plate method is recommended to use than pour plate method?
The bacterial colonies prepared by the pour plate technique is shown in figure 1. Bacterial growth of the spread plate only occurs on the surface of the plate. Therefore, spread plate technique gives well-separated colonies that are easy to count and pick up.
Who invented pour plate method?
Petri’s Famous Plate Let’s take a look back in time when Julius Petri was a military physician working in Robert Koch’s lab in Germany during the 1880’s. The current state of the art in the microbiology lab was to pour an agar based nutrient media into an open dish and place it under a bell jar.
Is pour plate used for isolation?
THE POUR PLATE AND SPIN PLATE METHODS OF ISOLATION. Another method of separating bacteria is the pour plate method. With the pour plate method, the bacteria are mixed with melted agar until evenly distributed and separated throughout the liquid. The melted agar is then poured into an empty plate and allowed to solidify …
Where are colonies formed during the pour plate method?
With the pour-plate technique, the colonies form within the agar as well as on the surface of the agar medium thus providing a convenient means to count the number of viable cells in a sample.
What is pour plate in microbiology?
In a pour plate, a small amount of inoculum from a broth culture is added by pipette to the centre of a Petri dish. Cooled, but still molten, agar medium in a test tube or bottle is then poured into the Petri dish.
Why do we use pour plate?
The most common method for determining the total viable count is the pour-plate method. The pour plate technique can be used to determine the number of microbes/ mL in a specimen. It has the advantage of not requiring previously prepared plates and is often used to assay bacterial contamination of foodstuffs.
How are microorganisms isolated in pour plate?
Which of the following accurately describes a successful pour plate?
Which of the following accurately describes a successful pour plate? Smooth agar surface with colonies on the surface and within the agar. Which of the following techniques results in isolated colonies found only in some areas on the agar surface?
Who developed pour plate method?
Robert Koch. ⇒ In Pour Plate technique, successive dilutions of the inoculum (serially diluting the original specimen of old broth culture) is added to the sterile Petri plates containing the melted and cooled (40-45 °C) agar medium & thoroughly mixed by rotating the plates which are then allowed to solidify.
What is the pour plate method?
The pour plate method is an economical way for pharmaceutical, contract and even food laboratories to perform tests focused on a specific number of bacteria. The process may seem simple (melt, pipette, pour, swirl, incubate), but errors have been known to occur.
How can I improve the success of my pour plate test?
Fortunately, there are several steps you can take to ensure the success of your test. Try implementing these 11 best practices the next time you use the pour plate method for growth promotion testing (GPT), microbial enumeration, or bioburden testing. 1. Keep the molten agar in the water bath for no more than three to four hours.
How do you prepare a sample for plate plating?
The samples may be derived from a dilution series of a single sample. The sample volume to be plated should be between 0.1 and 1.0 ml. Open the lid of the empty Petri dish, and dispense your sample into the middle of the plate. Close the lid. Use aseptic technique throughout this procedure.
What is the difference between streak plate and pour plate?
To insure a countable plate a series of dilutions should be plated. The pour plate method of counting bacteria is more precise than the streak plate method, but, on the average, it will give a lower count as heat sensitive microorganisms may die when they come contact with hot, molten agar medium.