What is ROSA26 locus?
Known in scientific circles as Gt(ROSA)26Sor, the Rosa26 locus is a specific genetic site that is located on mouse chromosome 6, and is considered to be one of the ideal locations where knock-ins of interest can be targeted.
What is the ROSA26 gene?
ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice. It was first isolated in 1991 in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 130 knock-in lines have been created based on the ROSA26 locus. The human homolog of the ROSA26 locus has been identified.
Is ROSA26 a promoter?
The activities of nine ubiquitous promoters (ROSA26, CAG, CMV, CMVd1, UbC, EF1α, PGK, chicken β-actin and MC1) have been quantified and compared in mouse embryonic stem cells.
What is the AAVS1 locus?
The adeno-associated virus site 1 (AAVS1) locus in the human genome is a strong candidate for gene therapy by insertion of an exogenous gene into the locus. The AAVS1 locus includes the coding region for myosin binding subunit 85 (MBS85).
What is the Rosa promoter?
ROSA promoter (Ubiquitous) in pDRIVE expression plasmid. The ROSA26 promoter, initially identified by random retroviral gene trapping in mouse embryonic stem cells [1], directs expression of reporter [2] and recombinase genes [3] in all cells throughout embryonic development and in adult tissues.
What is safe harbor locus?
The AAVS1 locus, in intron 1 of PPP1R12C (protein phosphatase 1 regulatory subunit 12C), is known as a “safe harbor” because its disruption does not have adverse effects on the cell, and robust transcription can be used to maintain the expression of an exogenously inserted gene5,6,7.
What is a ROSA26 mouse?
The ROSA26 allele is a defined locus on mouse chromosome 6 that has been used extensively for the targeted insertion of a wide variety of transgenes (Nyabi et al., 2009) including fluorescent protein reporters under transcriptional control of ubiquitously expressed housekeeping genes.
What is the human homolog of ROSA26 locus?
The human homolog of the ROSA26 locus has been identified. ROSA stands for R everse O rientation S plice A cceptor, named after the lentivirus genetrap vector.
How many knock-in lines have been created from the ROSA26 locus?
Over 130 knock-in lines have been created based on the ROSA26 locus. The human homolog of the ROSA26 locus has been identified. ROSA stands for R everse O rientation S plice A cceptor, named after the lentivirus genetrap vector.
What is the significance of the ROSA26 gene?
ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice. It was first isolated in 1991 in a gene-trap mutagenesis screen of embryonic stem cells (ESCs).
What is the rosaβgeo26 line?
ROSAβgeo26 (ROSA26) refers to a locus that is widely used for achieving generalized expression in the mouse. The ROSAβgeo26 (GtROSA26) line was initially derived from pools of ES cells infected with the retroviral gene trap vector Gen – ROSAβgeo at low multiplicity of infection ( Friedrich and Soriano, 1991 ).