What is SV40 ori?
The SV40 origin can be used to increase the level of transcription in cells that are transfected with the plasmid because the plasmid is replicated. It is also reported that by using selectable markers in conjunction with the SV40 origin stable cell lines can be produced.
How many origins of replication are in pBR322?
It contains the origin of replication of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number. The plasmid has unique restriction sites for more than forty restriction enzymes. Eleven of these forty sites lie within the TetR gene.
Can a plasmid have multiple ori?
Two different Ori helps the plasmid to replicate in different host organisms. Thus the same vector can be directly propagated from one host to another. Its is a type of shuttle vector.
What determines copy number of plasmid?
Plasmids vary widely in copy number depending on three main factors: 1) The ori and its constituents – (e.g. ColE1 RNA I and RNA II). 2) The size of the plasmid and its associated insert (bigger inserts and plasmids may be replicated at a lower number as they represent a great metabolic burden for the cell).
What is SV40 promoter?
The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and cellular factors that are involved in transcriptional control and initiation.
How many ori are in eukaryotes?
In contrast, eukaryotic genomes contain significantly more origins, ranging from 400 in yeast to 30,000–50,000 in humans (Cvetic and Walter 2005; Méchali 2010), because timely duplication of their larger linear chromosomes requires establishment of replication forks at multiple locations.
How many Ori do bacteria have?
Among bacteria, one replication origin is the norm and there is currently no evidence that two functional origins are ever used on the same chromosome. However, it seems that there are always exceptions to the rules of biological systems.
What is a high copy number plasmid?
A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture.
How are plasmid copy numbers regulated?
By controlling the synthesis of the Rep protein, the plasmid copy number can be controlled. The R1 plasmid uses its complementary RNA to inhibit the translation of the mRNA that encodes the Rep protein and inhibits the replication of the plasmid DNA.
How is SV40 hybrid DNA quantified using oripcr?
Hybrid DNA carrying SV40 Ori, SV40 early genes, and capsid protein genes from either JCV or LPV was similarly quantified using the OriPCR primers. BKV DNA was quantified using the primer set 5′-TCTTTCCCCTATTAGGCCCTCAATGG-3′, which hybridizes the BKV sequence 1166–1191, and 5′-CTCTCCTGAATGTACAGTTTGTTG-3′, which is complementary to 1325–1302.
What is simian virus 40 (SV40)?
Simian virus 40 (SV40) is a polyomavirus, a non-enveloped small DNA virus whose capsid is composed of major capsid protein Vp1 that forms a pentamer, 72 of which are arranged as an iosahedral capsid shell, and minor capsid proteins Vp2 and Vp3, one of which resides in the inner cavity of the Vp1 pentamer ( Imperiale and Major, 2007 ).
What does SV40 stand for?
Simian virus 40 (SV40) is a polyomavirus, a non-enveloped small DNA virus whose capsid is composed of major capsid protein Vp1 that forms a pentamer, 72 of which are arranged as an iosahedral capsid shell, and minor capsid proteins Vp2 and Vp3, one of which resides in the inner cavity of the Vp1 pentamer (Imperiale and Major, 2007).
How do I contact the SV40 gene editing team?
* To whom correspondence should be addressed. Tel: +49 2302 669 144; Fax: We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions.