What is the marker for macrophages?
Common cell surface markers used to identify human and mouse macrophages include CD11b/Integrin alpha M, CD14, CD68, Fc gamma RIII/CD16, Fc gamma RI/CD64, and CCR5, along with F4/80 in mouse. Additional markers are used to identify specific tissue-resident macrophages and different macrophage activation states.
Are macrophages CD45 positive?
CD45 is a protein tyrosine phosphatase expressed on all cells of hematopoietic origin that is known to regulate Src family kinases. In macrophages, the absence of CD45 has been linked to defects in adhesion, however the molecular mechanisms involved remain poorly defined.
What is CD15 a marker for?
The glycan determinant CD15 (also known as Lewis x, or Le(x)) is a distinguishing marker for human myeloid cells and mediates neutrophil adhesion to dendritic cells.
Is CD45 a macrophage marker?
The expression of the macrophage markers CD68, CD163 and CD11b, CD45 (a general leukocyte marker), MHCII (expressed by antigen-presenting cells [APCs]), CD11c (dendritic cell marker), lymphatic endothelium hyaluronan receptor-1 (LYVE1; expressed on lymphatic endothelium and macrophage subsets), chemokine receptor 7 ( …
What are the lineage specific rat markers?
Lineage Specific Rat Markers Cell Marker Antibodies (catalog #) Pan specific T cell CD3 MCA772 Helper T cell CD4 MCA55G Cytotoxic T cell CD8 alpha, CD8 beta MCA48G , MCA938GA Thymocytes CD90 MCA47G
How do you detect macrophages in blood cells?
Thereafter, erythrocytes were lysed and renal macrophages were detected by using flow cytometry. Cells were incubated with antibodies for CD45, CD86 and CD163 for membrane detection and CD68 for intracellular staining (Figure 1B). Finally, the phenotype of the macrophage populations was analyzed according to the scheme showed in Figure 1C.
What are the phenotypes of macrophages?
Macrophages may be classified into two different phenotypes: classically activated macrophages (M1 macrophages), that release pro-inflammatory cytokines and promote fibrosis; and alternatively activated macrophages (M2 macrophages) that are associated with immunoregulatory and tissue-remodeling functions.
What is the marker for multiple myeloid cells?
It is predominantly expressed on vascular endothelium, and also on bone marrow stromal cells, follicular dendritic cells and some macrophages. Rat markers CD11b and CD106 have been used in flow cytometry (Figure 3), showing myeloid cell populations.