What is the spot inoculation technique?

The spot inoc- ulation method involves the placement of a drop or spot of bacterial suspension onto a surface of produce with a mi- cropipette. Pathogens on spot-inoculated lettuce have been reduced by 1 to 4 log CFU/g; however, the amount of re- duction of pathogens among the published studies differs (15, 17, 20).

What is spot inoculation in microbiology?

A spot inoculation is used to ensure that a large amount of bacteria will grow at a single location and produce a concentrated amount of amylase.

What are the types of inoculation techniques?

Types of Inoculation

  • Direct Inoculation.
  • Covert Inoculation.
  • Indirect Inoculation.
  • Formal Inoculation.
  • Informal Inoculation.
  • Subliminal Inoculation.

What are different bacterial inoculation techniques?

Introduction of Bacterial Inoculation Method Different inoculation methods used in bacteriology are the streak plating technique, spread plate technique, and agar stab technique.

What is pour plate technique?

The pour-plate technique Molten agar cooled to 45°C, is poured into a Petri dish containing a specified amount of the diluted sample. Following the addition of the molten-then cooled agar, the cover is replaced, and the plates gently rotated in a circular motion to achieve uniform distribution of microorganisms.

Why do we inoculate bacteria?

Inoculation is transfer of a bacterial sample onto a growth media for the purpose of growing the sample. Of course, you must use aseptic technique to avoid contamination. Tools used for inoculation must be sterile. That is, there is nothing growing or alive on the tools.

What is streak plate technique?

In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.

What is streak plate?

An unglazed piece of porcelain, such as a tile, used to test the characteristic streak of minerals by rubbing the mineral across the tile. Streak plates have a hardness of about 6.5 on the Mohs scale and cannot be used for testing harder minerals.

What is an example of inoculation?

For example, an inoculation message designed to discourage teen cigarette smoking (e.g., Pfau et al., 1992) might begin with a warning that peer pressure will strongly challenge their negative attitudes toward smoking, then follow this forewarning with a handful of potential counterarguments they might face from their …